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KMID : 0545119910010040227
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 4 p.227 ~ p.231
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Molecular Cloning of β-Galactosidas from Bacillus subtilis HP- 4
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KIM, JEONG HO
LEE, JAE, CHANG/HUH, JEONG WON/CHUNG, KI CHUL/BAEK, YOUNG JIN/LEE, YOUNG KYU
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Abstract
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A gene coding for a β-galactosidase of Bacillus subtilis HP-4 was cloned in E. coli JM109 by inserting HindIII digested fragment of B. subtilis HP-4 chromosomal DNA into the site of pBR322 and selecting recombinant transformant showing blue color on X-gal plate. The recombinant plasmid, named pBG109, was found to contain the 1.4 Kbp HindIII fragment originated from B. subtilis HP-4 chromosomal DNA by Southern hybridization. The cloned gene was stably maintained and expressed in E. coli JM109 and the pBG109 encoded β-galactosidase had the same enzymatic properties as those of β-galactosidase produced by B. subtilis HP-4.
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